Melendez: The PI occupies a laboratory of approximately 1400 sq. ft. of floor space and 200 linear feet of bench. The PI’s lab is equipped with a sinks, chemical and tissue culture hoods, dishwasher, autoclave, microplate reader, ABI 7500 RT thermal cycler, Eppendorf mastercycler, water baths, rotary shakers, micro-centrifuges, a refrigerator/freezer, -80 freezers, UV/VIS spectrophotomoter and gel documentation system. The lab is also equipped with 3 laptop computers for data analysis and manuscript preparation. The PL’s tissue culture room contains two 6 ft laminar flow hoods, 4 Forma steri-cult incubator, 1 Forma low-gas steri-cult incubator and Nikon Diaphot 200 inverted microscope including bright field and epi-fluorescence capabilities, dichroic cube sets B-2E and G1-B, Nikon objective lens set including: CF Fluor 20x, O.75 n.a., CFN Plan Fluor 40x, 0.7 n.a., CFN 60x oil Plan Apochromat, 1.21 n.a., CFN 100x oil Plan Apochromat, 1.4 n.a. lenses.inverted microscope. The laboratory also has 2 Mettler top-loading balances, Savant Speed-Vac concentrator, Beckman dual-beam spectrophotometer, Biotek Synergy UV/VIS/Fluor/Chemiluminescence plate reader Gibco/BRL horizontal agarose gel units and polyacrylamide slab gel units, 10 LKB power supplies, Forma -80C freezer, 3 Freezers and 2 refrigerator/freezer, 2 Shaking incubators, 2 bacterial/yeast incubators, 2 Chemical hoods. Amnis ImageStream (Amnis Corporation) imaging flow cytometer that is equipped with 488 nm and an extended depth of field module. This instrument, which operates in a Windows-based environment, introduces a new technology platform that captures high-resolution digital images of cells in flow at rates of approximately 100 cells/per second. It simultaneously acquires up to six different images of each cell, including four colors of sensitive fluorescence imagery, side scatter and brightfield imagery. The ImageStream combines the quantification and analysis of cellular morphology with the ability to perform all the fluorescence intensity measurements of conventional flow cytometry, allowing for rapid analysis and classification of thousands of cells using the ImageStream IDEAS software. The IDEAS software calculates over 100 parameters per cell, including all the standard intensity-based parameters and statistics employed in flow cytometry as well as numerous morphological parameters such as cell area, perimeter, aspect ratio, texture, spot counts and internalization ratios. The ImageStream is ideally suited for quantitative investigations of nuclear translocation, phagocytosis, intracellular co-localization, apoptosis, cell: cell interactions and fluorescent in situ hybridization in suspension. Meso Scale Discovery SECTORTM Imager 2400 (SI2400) instrument that enables either a single or multiple analytes/biomarkers to be determined simultaneously within a single well using a small amount of sample. It has exceptional sensitivity, wide dynamic range and convenience using a line of custom microplates. The SI2400 instrument accommodates 96- and 384-well single-spot plates as well as 96-well multi-spot plates. This platform is ideal for large scale clinical studies and screening.The PI also has full access to a Zeiss AxioVert200M fully automated inverted microscope with motorized stage quipped for multicolour, multiposition live cell imaging and feature .live cell, phase Contrast, bright field, DIC, color camera, Z-stack and timelapse.
Computers:
Multiple Lenovo desktop computers with internet access are available, all equipped with appropriate software for data analysis, word processing, email, etc. Computers and infrastructure are supported by CNSE computer department. The PI and his students also have access to OriginLab, Statistica and GraphPad Prism (plotting and statistical analysis software); ANSYS Multiphysics, FLUENT, IntelliSuite and COMSOL (finite element analysis and simulation software); as well as L-Edit, Cadence, and K-Layout (lithographic layout software) that is hosted on the CNSE network and is supported by the CNSE Supercomputer Core Facilities.
Shared equipment: includes -150°C Revco, -80°C and -20°C freezers, +4°C refrigerators, acid fume hoods, four Thermo Scientific CO2 incubation chambers, four Class II/A2 biological safety cabinets,Tecan Infinite M200 microplate fluorometer with UV/VIS spectrophotometer capability, BioRad Protean II gel electrophoresis equipment, BioRad VersaDoc 4000 Imaging System, NanoDrop ND-1000, Spectrophotometer, NanoDrop ND-3300 Fluorospectrometer, Malvern Zetasizer Nano, centrifuges, autoclave, and a humidity controlled BioForce Nano eNabler (NeN) QPL instrument (Bioforce Nanosciences) for cell printing adjacent to cell incubators and fluorescent microscopes.
CNSE Microscopy and Analytical:
The CNSE complex also has a full suite of metrology tools including electron and ion microscopes (TEM/SEM/FIB), light microscopes, X-ray photoelectron spectroscopy, atomic force microscopes and other surface analysis and microscopy tools. Also available are a Bruker D8 ADVANCE X-ray Diffractometer (HR-XRD), Thermo VG Scientific Theta Probe X-ray Photoelectron Spectroscopy (XPS), Digital Instruments Nanoscope III Scanning Probe Microscope (SPM/AFM) Multimode and 3100 system with liquid cell, LEO 1550 Scanning Electron Microscope with a ThermoNoran Voyager Energy Dispersive X-ray Detector and a MaxRAY WDS system (SEM), JEOL 2010F High Resolution Transmission Electron Microscope (TEM) with EELS and a Nanofactory TEM-STEM holder, FEI Nova NanoLab 600 Dual Beam Focused Ion Beam/Scanning Electron Microscopy System (FIB/SEM), Nicolet 6700 FT-IR with transmission and diffuse reflection measurement capability. A J.A. Woollam InfraRed-Variable Angle Spectroscopic Ellipsometer (IR-VASE) is available for transmission and reflection measurements on thin films and monolayer chemistries. ICP: Perkin Elmer Nexion 300 ICP-MS, The Nexion 300 is a bench top ICP-MS (Inductively Coupled Plasma Mass Spectrometer) with a quadrupole mass analyzer that is capable of performing trace elemental analysis. The instrument was recently installed and will be an open access instrument. Users are granted access after receiving training.
Additional Resources available at UAlbany-SUNY Life Science Research Building Core Facilities:
Molecular Core Facility: includes a real-time PCR machine (ABI 7900HT), several traditional PCR machines (ABI), a low volume spectrophotometer (NanoDrop), centrifugues (from desktop models to high-speed ultracentrifuges), BioRad ChemiDox XRS, GE Typhoon Trio phosphoimager, BioRad Pathfinder FPLC, PCR/DNA sequencing, bacterial shakers, and basic molecular biology equipment and microscopes: MMI LCM microscope, Leica fluorescent dissecting microscope, Nikon TS100 microscope. Biological Imaging Facility: Includes a Zeiss 940 Digital SEM, Zeiss 902 TEM, Zeiss LSM510/META scanning head confocal microscope, and Zeiss AxioVert 100M inverted fluorescence microscope with 20X Plan ApoChromat (NA 0.75, WD 0.61), 40X AchroPlan (water, NA 0.8, WD 3.61 mm), 63X PlanApochromat (oil, NA 1.4, WD 0.18) and 100X PlanNeofluor (oil, 1.3, WD 0.17). Digital imaging includes Metamorph and NIH/Scion software; Hammamatsu ORCA CCD cameras, and high quality calcium-sensitive fluorescence imaging via Metafluor. Photography facilities are also present. Tissue Culture Core Facility: The tissue culture facility is a biosafety level 2 facility and contains basic tissue culture hoods, water-jacketed incubators, a liquid nitrogen cell freezer, and -80C freezers. Crystallography Facility: Bruker D8 Advance X-Ray powder diffractometer equipped with a Globel mirror and a Super Speed Linxeye positional detector, Bruker CCD Single Crystal X-Ray diffractometer equipped with a cooling device. Structural Chemistry Core: Fluorolog-3 fluorescence spectrometer, Lambda-950 UV/Vis/Near IR absorption spectrophotometer, Spectrum-100 FT-IR spectrometer, JASCO-400 stopped-flow instrument, Raman Microscope.
